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The role of MRE11 in activating cellular response after introduction of DNA double- strand breaks in Arabidopsis thaliana (L.) Heynh. (CROSBI ID 588915)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija

Šamanić, Ivica ; Cvitanić, Ratko ; Puizina, Jasna , Riha, Karel The role of MRE11 in activating cellular response after introduction of DNA double- strand breaks in Arabidopsis thaliana (L.) Heynh. // Book of Abstracts of the 3rd Congress of Croatian Geneticists with international participation / Franketić, Jasna ; Garaj-Vrhovac, Verica (ur.). 2012. str. 37-37

Podaci o odgovornosti

Šamanić, Ivica ; Cvitanić, Ratko ; Puizina, Jasna , Riha, Karel

engleski

The role of MRE11 in activating cellular response after introduction of DNA double- strand breaks in Arabidopsis thaliana (L.) Heynh.

The aim of this study was to investigate the possible role of MRE11 in the DNA damage signaling and cell cycle control in Arabidopsi thaliana. We have taken a new cytogenetic approach to analyze the mitotic activity and chromosomal abnormalities after DNA double strand breaks (DSBs) introduction. Inflorescences of A. thaliana were cut off and treated with bleomycin – mutagen known to induce the DSBs and mitosis were analyzed from pistil cells after 24, 48 and 72 hours recovery periods. We have monitored the behavior of three mre11 mutant lines that harbored T-DNA insertions in different parts of the gene and compared their response with wild-type (wt) and atm mutant line. The ATM protein is known to play a central role in cells' response to DNA damage. We obtained following results: 1) wt plants showed reduced mitotic activity coupled with extensive chromosome abnormalities after 24 hours of recovery, but mitotic activity reached nearly normal level after the 72 hours of recovery period, with also lower rate of chromosomal abnormalities 2) all three mre11 mutant lines showed no reduction in mitotic activities in spite of extensive chromosome fusions and fragmentation 3) atm mutant line mirrored the behavior of mre11 mutants. Obviously, in mre11 and atm mutants cell cycle arrest was abrogated and lesions were not properly repaired. These observations have been additionally confirmed by the results of RT- PCR. Bleomycin-treated mre11 and atm mutants in comparison with wt plants, had lower expression of genes involved in: cell cycle control (CYCB1 ; 1, WEE1, KNOLLE), DNA damage signaling (MRE11, ATM, ATR) and repair (RAD51, BRCA1, SYN2, PARP1). Taken together these results support our assumption that MRE11 gene is involved in DNA damage signaling and plant cell cycle control.

MRE11; Arabidopsis thaliana; Mitosis; DNA double-strand breaks; Cell cycle delay

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Podaci o prilogu

37-37.

2012.

objavljeno

Podaci o matičnoj publikaciji

Book of Abstracts of the 3rd Congress of Croatian Geneticists with international participation

Franketić, Jasna ; Garaj-Vrhovac, Verica

978-953-57128-0-0

Podaci o skupu

3rd Congress of Croatian Geneticists with international participation

predavanje

13.05.2012-16.05.2012

Krk, Otok Krk, Hrvatska

Povezanost rada

Biologija