engleski

Immunopathological Response Against Trichinella spiralis in Mice

By the first quantitative phenotypic analysis of intestinal intraepithelial lymphocytes (i-IEL) at the early stages of theTspiralis infection, we found that the kinetic of ((T cells in i-IEL corresponded to the kinetics of worm expulsion after infection of C57BL mice with 200 muscle larvae. Infected mice developed signifcant enteropathy, comprising villus atrophy, crypt hyperplasia, goblet cell hyperplasia and a decrease in i-IEL numbers by 14 days post infection (p.i.), when most of the parasites had been expelled. Most murine i-IEL of the gdT cell lineage tend to be cytolytic when activated.We speculated that gdT cells of i-IEL during the early stages of infection recognize and eliminate damaged epithelial cells generated by parasite antigens, simultaneously accelerating the worm expulsion. However, since the rejection ofT.spiralis worms is dose-dependent effect, to assess whether or not i-IEL, particularly gdT cells, respond comparably to different sized T.spiralis infection, we used C57BL mice, orally infected with 50 or 400 viable T.spiralis larvae. We found that the rapid activation of gdT cells in i-IEL occured much sooner in mice infected with 400 larvae than in mice infected with 50 worms, as early as 1 day p.i. vs 4 day p.i., respectively In addition we found significant elevations of eosinophiles and mastocytes on day 1 p.i. in mice infected with 400 T.spiralis larvae in comparision with mice infected with 50 larvae. In spite their intrinsic cytolytic activity against transformed cells, no corelation between kinetics of gdBT cells in i-IEL and T.spiralis worm expulsion was observed in mice infected with 400 or 50 Iarvae.These fndings strongly suggest that bothTspiralis infection levels elicits immunodepression by interFerence with the first line defenceT cell function, therefore influence the outcome of experimental T.spiralis gut infection.

Trichinella spiralis; mice

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