engleski

In-vitro assessment of oxidative stress generated by orthodontic archwires

Several metals undergo redox cycling, producing free radicals and generating oxidative stress. The purpose of this study was to investigate in-vitro oxidative stress of orthodontic archwires made of various alloys. Mouse fibroblast cells L929 were exposed to 6 types of archwires, and the concentration of the oxidative stress marker 8-hydroxy-2'- deoxyguanosine in DNA was evaluated. Trypan blue dye was used in the determination of cell viability and numbers. Standard nickel- titanium archwires generated the highest oxidative stress, significantly higher than all other wires and the controls (P <0.05), and coated nickel- titanium, copper-nickel-titanium, and cobalt- chromium were lower than nickel-titanium (P <0.05), but higher than titanium-molybdenum and the negative and absolute controls (P <0.05). Titanium-molybdenum and stainless steel generated the lowest stress. Nickel-titanium induced the lowest viability, lower than the negative and absolute controls and all other wires (P <0.05) except titanium-molybdenum. Stainless steel showed the highest viability. Nickel-titanium produced the highest inhibition of cell growth, higher than all samples (P <0.05) except the positive control and cobalt-chromium. The lowest inhibition was observed in stainless steel and titanium- molybdenum, lower than nickel-titanium, cobalt- chromium, and the positive control (P <0.05). All orthodontic archwires generate oxidative stress in vitro. Stainless steel archwires have the highest and nickel-titanium the lowest biocompatibility.

oxidative stress; genotoxicity; viability; orthodontic archwires

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