engleski

Mitochondrial genetic variation in two Podolian cattle breeds in Croatia

Two Croatian endangered autochthonous cattle breeds, Istrian cattle and Slavonian-Syrmian podolian cattle are included in conservation program and insight in their genetic structure is a basic prerequisite of successful conservation strategies. Mitochondrial DNA represents an informative genetic marker in knowing the genetic structure of the populations. The aim of research was evaluation of genetic variability in two local Podolian cattle breeds based on analysis of the mtDNA D-loop region from 25 animals. Sequencing the 785-bp fragment of mtDNA D-loop region revealed 23 polymorphic sites representing 15 different haplotypes which were clustered into five haplogroups. In sequences of Slavonian- Syrmian Podolian cattle six polymorphic sites were found and in sequences of Istrian cattle 21. The nucleotide sequence diversity in Slavonian-Syrmian Podolian cattle was lower than in population of Istrian cattle, 1.78% and 3.03%, respectively. In Slavonian-Syrmian Podolian population were observed three haplogroups of which two are observed in the population of Istrian cattle. Overall mean of genetic distances in Slavonian- Syrmian Podolian cattle was lower (0.002) than in population Istrian cattle (0.004). Coefficient of differentiation in entire population was 0.081. The high numbers of identified haplotypes in population of Istrian cattle indicate a high genetic variability of the maternal component, while in population of the Slavonian-Syrmian Podolian cattle lower genetic variability was observed. Our results show a relatively moderate genotypic diversity within and between the two analyzed cattle breeds. The genetic diversity information based on mtDNA is important baseline data for the future breed conservation strategy.

cattle breeds; mtDNA; haplogroups; conservation strategies

nije evidentirano