Oxidation of N-Cbz-aminopropanol catalyzed by alcohol dehydrogenase (CROSBI ID 589692)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Janžetić, Kristina ; Sudar, Martina ; Findrik Blažević, Zvjezdana ; Vasić-Rački, Đurđa
engleski
Oxidation of N-Cbz-aminopropanol catalyzed by alcohol dehydrogenase
Alcohol dehydrogenases (ADHs) catalyze oxidoreduction reactions of a broad spectrum of substrates, i.e. alcohols, aldehydes and ketones [1]. Therefore they attract great scientific interest [2]. They play an important role in several physiological functions (in plants, microorganisms and animal tissues) including metabolism of alcohol where they catalyze the reversible oxidation of alcohols to corresponding aldehydes or ketones with the use of coenzymes [3]. In this work the oxidation of N-Cbz-3- aminopropanol (Scheme 1) was investigated. Scheme 1. The reaction scheme Two different enzymes were used for the oxidation of this alcohol, alcohol dehydrogenase isolated from Saccharomyces cerevisiae and alcohol dehydrogenase from horse liver. Since ADH requires coenzyme, NAD+, for its catalytic activity, two different NAD+ regeneration systems were investigated and compared: the addition of cosubstrate - acetone and the addition of NADH oxidase (NOX) isolated from Lactococcus lactis. The product of this reaction, N-Cbz-3- aminopropanal, is the substrate for the reaction of aldol addition catalyzed by D-fructose-6- phosphate aldolase (FSA), and these two reactions were coupled in order to avoid the isolation and purification of the aldehyde. All reactions were carried out in a batch reactor.
alcohol dehydrogenase; coenzyme regeneration; aldola addition
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Podaci o prilogu
8-8.
2012.
objavljeno
Podaci o matičnoj publikaciji
Vrsalović Presečki, A. ; Vasić-Rački, Đ.
Zagreb: Fakultet kemijskog inženjerstva i tehnologije Sveučilišta u Zagrebu
978-953-6470-59-4
Podaci o skupu
Applied Biocatalysis, 8th meeting of students and university professors
predavanje
19.09.2012-19.09.2012
Zagreb, Hrvatska