engleski

Aldol addition catalyzed by two different D- fructose-6-phosphate aldolases

Aldolases belong to a group of enzymes called lyases and they catalyze carbon-carbon bond formation [1]. In this work aldol addition of dihydroxyacetone (DHA) to N-Cbz-3-aminopropanal (aldehyde) was studied (Scheme 1). The aldol product of the reaction studied in this work is used as precursor in the production of fagomine, a naturally occurring iminosugar with remarkable biological properties [2]. Scheme 1. The reaction scheme The reaction investigated in this work was catalyzed by D-fructose-6-phosphate aldolase from genetically modified E. coli. Two different aldolases were investigated, FSA A129S and FSA A129S/A165G. In order to determine the best conditions for this reaction, different types of reactors were used. The reaction of aldol adition was carried out in a batch reactor, in enzyme membrane reactor and in a microreactor.

aldol addition; D-fructose-6-phosphate aldolase

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