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Genotoxic biomonitoring of flowable and non-flowable composite resins in peripheral blood leukocytes

Composite restorative materials represent one of the most important groups of materials in contemporary dental practice. However, their incomplete polymerization may lead to monomer induced genotoxicity. The objective of this study was to evaluate the genotoxicity of three flowable (Filtek Supreme XT Flow, Tetric EvoFlow, Gradia Direct Flo) and three non-flowable dental composite materials (Filtek Z250, Tetric EvoCeram, Gradia Direct Posterior). Genotoxicity assessment of composite materials was carried out in vitro in human peripheral blood leukocytes using the alkaline single-cell gel electrophoresis technique (comet assay). Prepared materials were eluted in saline solution for 1 h, 1 d, and 5 d. Thereafter leukocyte cultures were treated with different concentrations of eluates obtained from each of tested dental composite materials. Kruskall-Wallis non parametric test was used for statistical analysis (p<0.05). The tested materials did not show genotoxic effects after exposure of leucocytes to 1 h eluates. Culture treated with 1 d eluates of all tested materials, only at a highest concentration (10-2) affected the measured cytogenetic parameters. Of all tested materials, only Filtek Z250 and Filtek Supreme XT Flow did not exhibit a genotoxic effect in cultures that were under the influence of 5 d eluates. Tested materials exhibited limited genotoxic activity in peripheral blood leukocytes. Since the effect was observed only in leukocyte cultures treated by one-day eluates at the highest concentration (10- 2) and it decreases in cultures exposed to 5 d eluates, it should not pose a significant risk to the human genome.

biocompatibility; comet assay; composite resin; genotoxicity test; leukocytes

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