engleski

The effects of silicon on the species Polycelis felina (Daly.) (Platyhelminthes, Turbellaria, Tricladida) in laboratory conditions

Silicon is one of the most frequent elements in the Earth's crust. It is an essential element in lower life forms. The effect of silicon on aluminium intake in the body was investigated more recently. Presence of silicon in plant cell culture reduces the death rate compared to cells treated only with aluminium. Small amounts of silicon have a positive effect on the proliferation and differentiation of human osteoblasts while higher concetrations stimulate osteoblasts to cell death. Some authors reported that higher concentrations of silicon damage DNA, and is therefore used in vitro as an inducer of cancer. We analyzed the effect of sublethal concentrations of silicon (0.17 g/dm3, 0.20 g/dm3, 0.23 g/dm3, 0.25 g/dm3, 0.27 g/dm3, 0.30 g/dm3) on Polycelis felina (Daly.) in laboratory conditions. Animals were left in testsolutions for 24 hours at 4 degree. After single exposure animals were transferred into clean aquarium water for recovery and were observed following 15 days. It has been established that sodium metasilicate, depending of dosage of applied concentration, causes mortality, decephalization, depigmentation morphological and cyto - histological changes. Mortality rate and the most intensive morphological and cyto - histological damages were established on the second and third day after the treatment. On histological slides of treated planarians were visible damages of basal membrane and parenchymal cells. Using HPLC a great amount of silicone was found in the body tissue so we concluded that silicon in increased doses had a damaging effect on Polycelis felina (Daly.).

sodium metasilicate (silicon) ; decephalization ; depigmentation ; regeneration

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