engleski

Antimutagenic effect of indole glucosinolates from Cruciferae family on 2-aminoanthracene induced mutagenicity in prokaryotic test-system

Glucosinolates are secondary plant metabolites widely distributed in plants of Cruciferae family (e.g., Brussels sprouts, a broccoli, cauliflower, cabbage). It has been shown that these natural plant compounds can be easily hydrolyzed to produce a range of physiologically active agents (isothiocyanates, nitriles and thiocyanates). Some of them show a significant antimutagenic/anticarcinogenic effects. The aim of our study was to investigate cytotoxic, mutagenic and antimutagenic effect of the indole-3-carbinol (I3C) and an extract of glucosinolates from cauliflower (CY). These substances are normally produced by hydrolysis of 3-indolylmethyl glucosinolate. Mentioned glucosinolate is broken down a through hydrolysis catalyzed by myrosinase, an enzyme released from damaged plant cells. In order to investigate the potential cytotoxic, mutagenic and antimutagenic effects of these substances, we used bacteria Salmonella typhimurium strain TA98 with metabolic activation. These investigations are based on several different test-protocols according to Dashwood, DeFlora and Preobrazhenskaya (6, 7, 18), including our modifications. The results showed that I3C has neither cytotoxic nor mutagenic effect, but it has significant antimutagenic effect. Furthermore, the results showed that CY caused a weak cytotoxic effect, but only at the highest concentration. In our mutagenic and antimutagenic investigations we used only noncytotoxic doses, where the survival of bacteria was up to 90%. The obtained data showed that CY had no mutagenic effect at any applied dose. On the other side, this glucosinolates-rich extracts successfully inhibit the action of 2-aminoanthracene (2-AA).

mutagenicity; antimutagenicity; Salmonella typhimurium; indole glucosinolates

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