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Biosynthesis and transport of salicylic acid in Arabidopsis thaliana infected by Plasmodiophora brassicae

Salicylic acid (SA) is a defense hormone required for the induction of plant resistance to pathogens. SA biosynthesis pathways and the type of its mobile form associated with induced resistance are plantpathogen specific. The clubroot disease is one of the most devastating diseases affecting all the members within the plant family Brassicaceae by causing serious losses of vegetable crops worldwide. In this study we wanted to investigate 1. which pathway of SA biosynthesis is implicated in the clubroot plantpathogen interaction and 2. whether methyl-salicylate (MeSA) is a mobile form of SA in this system. We have chosen Arabidopsis thaliana as a host organism for Plasmodiophora brassicae – the causal agent of clubroot, because the infection process is fast and the resistance of A. thaliana to P. brassicae is conferred by a small number of genes. Using isotope feeding and GC-MS analysis, we monitored the SA biosynthesis pathway as well as transport and metabolism of exogenously applied deuterated SA and its derivative, deuterated MeSA, through the whole plant of healthy and P. brassicae-infected A. thaliana. The results showed that in A. thaliana clubroots 1. SA is synthesized from chorismate and 2. MeSA is the preferred mobile form of SA.

Arabidopsis thaliana; Plasmodiophora brassicae; salicylic acid; biosynthesis; transport

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