engleski

Urine ochratoxin A and ochratoxin alpha in pregnant women from Croatia

This study determined exposure of pregnant women to ochratoxin A (OTA). Forty samples of fi rst- void urine samples from Croatian women in the third trimester of pregnancy were analysed for OTA and its major metabolite ochratoxin alpha (OTα). The subjects fi lled a short food frequency questionnaire (FFQ). Analysis was performed by HPLC-FLD following liquid-liquid extraction. All samples were subjected in parallel to enzymatic treatment (β-glucuronidase / aryl sulfatase) to release OTA and OTα from the conjugates. The median urinary levels of OTA and OTα before treatment were 0.02 ng mL-1 [range: (nd to 1.07) ng mL-1] and 0.16 ng mL-1 [(nd to 1.86) ng mL-1]. The concentrations after enzyme hydrolysis were 0.02 ng mL-1 [(nd to 1.11) ng mL-1] and 1.18 ng mL-1 [(0.11 to 7.57) ng mL-1]. While OTα levels increased signifi cantly following enzymatic treatment, evidence for OTA conjugation was weak. The ratio of urinary OTα medians after and before hydrolysis was 1.5 times higher than previously reported for nonpregnant female subjects, possibly indicating upregulated metabolism and/or elimination of mycotoxins and metabolites in pregnancy. The mean daily dietary OTA intake calculated from FFQs [(1.08±0.57) ng kg-1 b. w.] was well below the provisional tolerable daily intake and the greatest contributors to intake were cereal products, fruit juices, chocolate, and coffee.

detection of ochratoxins ; HPLC-FLD method ; mean daily dietary intake ; pregnancy

nije evidentirano