engleski

Oxidative stress in Sprague-Dawley healthy rats that underwent hyperbaric oxygenation

Oxidative stress is caused by an imbalance between the production of reactive oxygen species and a biological system's ability to readily detoxify the reactive intermediates or easily repair the resulting damage. The aim of this study was to investigate whether intermittent hyperbaric oxygenation (HBO) causes an increase of oxidative stress in male and female rats and whether there are differences in the amount of stress between the sexes. Healthy male (N=6) and female (N=6) Sprague-Dawley rats 10 weeks old were housed doubly in shoebox style cages with free access to standard rat chow and tap water, maintained on a 12:12 hour light: dark cycle. The animals were divided into control group and HBO group. Rats from the HBO group were treated in a hyperbaric chamber with 100% O2 for two hours a day during four consecutive days.[1] On the fifth day, arterial blood samples were collected to determine Ferric reducing ability of plasma(FRAP) and Thiobarbituric Acid Reactive Substances (TBARS ; based on reaction of malonilaldehyde (MDA) with thiobarbituric acid). Student t-test was used for statistical analysis. Male control rats have significantly higher FRAP than female controls (FRAP mM 0.05±0.03, 0.16±0.02, P<0.001), while there was no difference in TBARS between sexes in the control group (TBARS μM 0.56±0.37, 0.27±0.08, P=0.132). Four days of HBO therapy did not cause any significant changes in FRAP or TBARS in male rats compared to male controls, whereas TBARS were significantly higher after HBO therapy in female rats compared to female controls with no changes in FRAP. The results of this study may indicate that male rats have higher level of oxidative stress in control conditions, while four days of HBO therapy caused increase of oxidative stress only in female rats. Further studies are needed to conclusively interpret differences in maintaining oxidative balance between sexes in control conditions and during exposure to HBO therapy.

oxidative stress; HBO therapy; FRAP; TBARS

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