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IMPAIRED INTERNODAL IONIC CONDUCTANCES IN MYELINATED AXONS OF PERIPHERAL NERVES FROM TRANSGENIC MICE WITH DIFFERENT NEUROFILAMENT EXPRESSION (CROSBI ID 478449)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Križ, Jasna ; Zhu, Q. ; Meyer, J. ; Simonić, Ante ; Julien, J.P. ; Padjen, Ante L. IMPAIRED INTERNODAL IONIC CONDUCTANCES IN MYELINATED AXONS OF PERIPHERAL NERVES FROM TRANSGENIC MICE WITH DIFFERENT NEUROFILAMENT EXPRESSION // FIFTH IBRO WORLD CONGRESS OF NEUROSCIENCE : Abstracts. 1999. str. 71-x

Podaci o odgovornosti

Križ, Jasna ; Zhu, Q. ; Meyer, J. ; Simonić, Ante ; Julien, J.P. ; Padjen, Ante L.

engleski

IMPAIRED INTERNODAL IONIC CONDUCTANCES IN MYELINATED AXONS OF PERIPHERAL NERVES FROM TRANSGENIC MICE WITH DIFFERENT NEUROFILAMENT EXPRESSION

Neurofilaments (NFs) are the major cyoskeletal component in large myelinated axons of the peripheral nervous system, and are composed of three prolein subunits, NF-L, NF-M and NF-H, with molecular weight of approximately 70 kD, 145 kD and 200 kD, forming the rope like structures. NFs play a critical role in determining axonal caliber and consequently conduction velocity of large myelinated fibers. The aim of this study was to examine the electrophysiological properties of myelinated axons with altered morphology in transgenic mice with different NF-H expression (NF-H knockout mice and mice overexpressing human NF-H, line 200 mice). Single axons membrane properties were measured on isolated sciatic nerves in vitro using intracellular standard current-clamp technique. Transgenic animals showed several functional deficits in physiological properties. In NF-H knockouts voltage responses revealed increased steady state input resistance (dRss) during depolarization (12.1 ą 1.0 Mohm control vs. 15.5 ą 1.0 Mohm ) and increased peak resistance during evoked hyperpolarization (hRp) (33.5 ą 2.4 Mohm, control vs. 42.6 ą 3.9 Mohm) (control n=28, NF-H-/- n=14). Axons from line 200 transgenic mice showed significant increase in peak (dRp) (14.2 ą 0.95 Mohm, control vs. 23.11 ą 1.7 Mohm) and dRss (12.07 ą 0.82 Mohm vs. 20.44 ą 1.12 Mohm) (control n=28, line 200 n=15) during depolarization associated with impaired inward rectification . Our studies demonstrate that targeted NF-H disruption or human NF-H hyperexpression in transgenic mice affect functional characteristics of large myelinated fibers ( ion channel defects ), suggesting a complex structure/function relationship distinct from previously established structural role of NF.

myelinated axons; transgenic mice; neurofilaments

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o prilogu

71-x.

1999.

nije evidentirano

objavljeno

Podaci o matičnoj publikaciji

FIFTH IBRO WORLD CONGRESS OF NEUROSCIENCE : Abstracts

Podaci o skupu

FIFTH IBRO WORLD CONGRESS OF NEUROSCIENCE

poster

11.07.1999-15.07.1999

Jeruzalem, Izrael

Povezanost rada

Kliničke medicinske znanosti