engleski

Comparison of intraplasmid rearrangements in Agrobacterium tumefaciens and Escherichia coli

In this work we have constructed an assay plasmid to study intramolecular recombination efficiency in Agrobacterium tumefaciens and compare it to that in Escherichia coli. The assay plasmid used contains direct repeats of incomplete spectinomycin genes that share a 570 bp region of homology and are separated by the ampicillin resistance gene. It enabled us to directly follow generation of deletion and amplification products in A. tumefaciens for the first time. We show here that predominantly RecA-independent mechanism contributed to formation of deletion and amplification products between direct repeats in A. tumefaciens. At the same time, deletion and amplification products were detected at similar frequencies, suggesting that dimer formation accompanies deletion reaction probably by a similar intramolecular event.

RecA ; Intramolecular recombination ; Agrobacterium tumefaciens ; Escherichia coli

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