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Evaluation of oxidative status and baseline DNA damage frequency in healthy female volunteers

It is known that the exposure to various environmental mutagens increase production of reactive oxygen species (ROS). The increase of ROS production with the consequent disturbance of the oxidative balance in the cell, called oxidative stress, perturbs the metabolism of macromolecules. As such, oxidative stress leads to various protein, lipid and DNA modifications that can eventually lead to cell insult and increase of disease risk in humans. The main antioxidant in the organism is glutathione (GSH) that plays central role in defense against different cell insults. Thus, the cellular level of GSH concentration depends on the exposure to oxidative stress. The aim of this study was to evaluate oxidative status through evaluating GSH and malondialdehyde (MDA) level and baseline frequency of DNA damage in young female volunteers. Peripheral blood and urine samples intended for the study were taken from the volunteer blood donors, representing general population and were of the similar age and life-styles. All of them were healthy at the moment of blood and urine sampling and interviews. They were not occupationally exposed to genotoxic agents. None of them reported alcohol consumption, drugs intake, the presence of known inherited genetic disorders or chronic diseases. For the six month period prior to the blood sampling subjects had not been exposed to ionizing or non-ionizing radiation for diagnostic or therapeutic purposes. The concentration of GSH in blood as measure of body antioxidative status and level of MDA in the urine as indicator of oxidative damage to the lipids was measured. Additionally, to evaluate baseline frequency of DNA damage alkaline version of the comet assay was applied. Presented results will give an inside into oxidative status and its relation to DNA damage frequency of healthy female volunteers which can serve as baseline values for further biomonitoring assessment.

oxidative stress, oxidative status, reactive oxygen species, peripheral blood, urine, lymphocytes, glutathione, malondialdehyde, baseline DNA damage

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