engleski

SSR profile survey of winter and spring barley compared to malt quality data

In this research we identified certain analogy between SSR polymorphism of referent foreign quality varieties (Vanessa – winter type and Scarlett – spring type) with two varieties of winter (Zlatko and Barun) and two of the spring barley types (Fran and Matej) from the Agricultural Institute Osijek breeding program. Malt quality characteristics such as malt extract, viscosity and friability were run through three consecutive years (2004, 2005 and 2006) at two locations (Osijek and Nova Gradiska). The correlation between SSR polymorphism and malt quality measurement values has been examined in order to attain certain level of marker diagnostic ability. Seven SSR markers previously described in literature as potential detectors of certain chromosomal regions of interest were used, as follows: malt extract (EBmac415 and EBmac501), viscosity (Bmag211 and Bmag337) and friability (HVM40, Bmag120 and Bmag7). According to quality data in general, spring barley cultivars when compared with winter cultivars had significantly higher malt extract content (80.57 ; 79.65), lower malt viscosity (1.588 ; 1.682), higher friability (52.61 ; 65.88) together with specific interactions with growing conditions. This distinction between winter and spring types of barley in a sense of genotype correlates to UPGMA dendrograms for observed traits, related markers and also to variability within observed germplasm (foreign vs. domestic varieties).

Barley; breeding; polymorphism; SSR; quality

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