engleski

Isolation and properties of mitochondrial aspartate aminotransferase from red muscle of grey mullet, Mugil auratus Risso

Following the chromatographic separation of the grey mullet red muscle extract, enzyme with aspartate aminotransferase activity was isolated. The isolated enzyme was a dimeric protein composed of identical subunits with the overall Mr of about 65000. According to its subcellular distribution, susceptibility to inhibitors, molecular and catalytic properties the isolated enzyme belongs to the mitochondrial form of aspartate aminotransferase.

aspartate aminotransferase; mitochondrion; enzyme purification; enzyme characterization

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