engleski

SUBCELLULAR LOCALIZATION OF ARABIDOPSIS THALIANA BPM1 PROTEIN IN BY2 AND ONION CELLS AFTER TRANSIENT TRANSFORMATION

Proteins with BTB/POZ and MATH domains recruit substrates for ubiquitination by largest E3 ligase Cul3 complex that play an essential role in the regulation of many biologic processes. A BTB/POZ-MATH gene family in Arabidopsis thaliana (BPM)comprises of 6 members. In these report dynamics of subcellular localization of eGFP labeled BMP1 after transient transformation of BY2 and onion cells wilbl e presented. Four hours after transformation BPM1:eGFP was localized exclusively in the cytoplasm. Within next 12 hours protein accumulates in nucleus but part of it still remains in cytoplasm. After 24 hours, protein is localized exclusively in nucleus, in speckle-type form. Two days after transformation BPM1:eGFP signal completely disappears from cells. In addition, A. thaliana plants were transformed with BPM1 gene under constitutive promoter, and although they transcribe transgene no protein was detected. The further characterization of BPM1 protein is under study and the future work will be focused on protein function.

MATH-BTB; protein localization; transient transformation; BPM1

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