Modulation of perforin expression in the decidual and peripheral blood cytotoxic lymphocytes in culture (CROSBI ID 90855)
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Podaci o odgovornosti
Štrbo, Nataša ; Laškarin, Gordana ; Sotošek, Vlatka ; Randić, Ljiljana ; Podack, Eckhard R. ; Rukavina, Daniel
engleski
Modulation of perforin expression in the decidual and peripheral blood cytotoxic lymphocytes in culture
PROBLEM: Perforin (P) is a cytolytic molecule located in intracellular granules of cytotoxic lymphocytes both in the peripheral blood and decidua of pregnancy. The aim was to analyze the kinetics of P expression during in vitro culture and modulation of P expression by adherent cells, their supernatants and mitogen (PHA) stimulation. METHOD OF STUDY: P (intracellular antigen) was detected by flow cytometry in the suspension of first trimester pregnancy peripheral blood lymphocytes (PBL) and decidual lymphocytes (DL). RESULTS: A decrease of the percentage of P+ cells was obtained after 1 hr incubation and was prevented by addition of 30% of decidual adherent cells (DAC) or their supernatants. Upregulation of P expression was obtained when, in addition to adherent cells, DL and PBL were stimulated by PHA. DAC present in the culture in physiological concentrations prevent downregulation of P expression. CONCLUSION: DAC located in the vicinity of decidual cytotoxic lymphocytes, owing to their unique ability to produce a wide range of substances on demand, contribute to the high level of P expression in the decidua of pregnancy.
Lymphocytes ; Decidua ; Peripheral blood ; Natural killer cells
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Podaci o izdanju
42 (1)
1999.
14-21
objavljeno
1600-0897
10.1111/j.1600-0897.1999.tb00460.x