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Investigation of the Flavonoids and Phenolic Acids of Sambuci flos by Thin-Layer Chromatography

The efficiency of ten thin-layer chromatographic (TLC)systems for the separation of flavonoids and phenolic acids identified in a methanolic extract of Sambuci flos has been investigated by three mathematical techniques: calculation of the information content derived from Shannon's equation ; determination of discriminating power (DP) and formation of clusters and dendrogram. The most suitable mobile phases for TLC of the compounds investigated are ethyl acetate-methanol-formic acid-water, 100+13.5+2.5+10 (v/v) and ethyl acetate-formic acid-water, 8+1+1 (v/v)

Sambuci flos; Flavonoids; Phenolic Acids; TLC; Numerical methods

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