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izvor podataka: crosbi

Protein and glycoprotein patterns related to morphogenesis in horseradish tissue culture (CROSBI ID 188232)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Peharec Štefanić, Petra ; Smolko, Ana ; Horvatić, Anita ; Cindrić, Mario ; Krsnik- Rasol, Marijana ; Balen, Biljana Protein and glycoprotein patterns related to morphogenesis in horseradish tissue culture // Croatica chemica acta, 86 (2013), 1; 27-37. doi: 10.5562/cca2078

Podaci o odgovornosti

Peharec Štefanić, Petra ; Smolko, Ana ; Horvatić, Anita ; Cindrić, Mario ; Krsnik- Rasol, Marijana ; Balen, Biljana

engleski

Protein and glycoprotein patterns related to morphogenesis in horseradish tissue culture

In this study cellular proteins of in vitro grown horseradish (Armoracia lapathifolia Gilib.) tissues of different morphologies, leaves (L), teratoma (TM) and tumor (TN), were separated by one-dimensional (1-DE) and two- dimensional polyacrylamide gel electrophoresis (2-DE) in order to reveal developmentally specific proteins. Moreover, proteins were subjected to analyses by lectin assays to reveal whether the morphological differences were reflected in protein glycosylation. 1-DE glycoprotein pattern showed substantial distinctions between horseradish tissues of different morphologies, which was in contrast to silver stained protein profiles, where only slightly qualitative changes were observed. 2- DE revealed more differences in expression of cellular proteins between examined tissues in comparison to 1-DE, while 2-DE blots confirmed quantitative difference in expression of 43 kDa glycoprotein between investigated tissues. Mass spectrometry analysis identified this protein as a typical horseradish peroxidase. Obtained results imply that the oligosaccharide structures could be important determinants of specificities of horseradish tissue morphology.

morphogenesis ; horseradish ; glycoproteins ; lectine assay ; tissue culture ; 2-DE

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Podaci o izdanju

86 (1)

2013.

27-37

objavljeno

0011-1643

1334-417X

10.5562/cca2078

Povezanost rada

Biologija

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