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Selection of an optimal set of solvents in thin-layer chromatography of flavonoids and phenolic acids of Lavandulae flos

A strategy for a rapid selection of a set from eleven thin-layer chromatographic (TLC) systems for the separation of flavonoids and phenolic acids identified in the methanolic extract of Lavandulae flos is disscused. For the selection of the optimal combination of systems, two approaches are possible. The first one is to determine and compare an amount of information and discriminating power for all possible combinations of TLC systems. The second approach is to classify the TLC systems into groups with similar separation properties and to select the best system from each group. The classification is carried out by numerical taxonomy methods. It has been shown that the most favourable TLC systems for the separation of investigated compounds are: ethyl acetate-formic acid-acetic acid-water (100:11:11:27 v/v) and ethyl acetate-formic acid-water (8.1:1 v/v)

Lavandulae flos; TLC; flavonoid; phenolic acid; numerical taxonomy

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