ADH based NAD+ regeneration in a microreactor (CROSBI ID 188317)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Šalić, Anita ; Ivanković, Mia ; Ferk, Elvira ; Zelić, Bruno
engleski
ADH based NAD+ regeneration in a microreactor
The coenzyme NAD+ (nicotinamide adenine dinucleotide) is commonly used in biocatalytic oxidations catalysed by the enzyme alcohol dehydrogenase (ADH). As the price of the coenzyme NAD+ is extremely high, it is essential to regenerate the reduced form of the coenzyme back into the oxidized form. In this paper the regeneration of the coenzyme NAD+ was carried out in a microreactor by reversible oxidation of ethanol to acetaldehyde with the ADH enzyme. A 100 % conversion of NADH was achieved for a residence time of just τ = 0.8 s when the concentration of acetaldehyde was in excess (ci, NADH = 5.5 mmol/dm3, ci, acetaldehyde = 44 mmol/dm3, γi, ADH = 0.2 g/dm3, 75 mmol/dm3 glycine-pyrophosphate buffer pH = 9 ; T = 25 °C). A 2D mathematical model for the description and prediction of microreactor performance was developed. Model simulations were validated using set of data from independent experiments. Due to high conversions that were obtained for short residence times, microreactors could be considered as good and efficient toll for coenzyme regeneration.
NAD+ ; coenzyme regeneration ; microreactor ; mathematical modelling
Ovaj se tekst temelji na radu koji je financirala Hrvatska zaklada za znanost.
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Podaci o izdanju
88 (9)
2013.
1721-1729
objavljeno
0268-2575
10.1002/jctb.4024