engleski

Genetic polymorphism of codling moth in Croatia assessed by SSR markers

This paper describes the effectiveness of SSR markers in assessing genetic polymorphism of codling moth (Cydia pomonella L.) sampled in Croatia. For this purpose nearly equal number of male codling moth individuals (total n=86) have been trapped both in conventional and organic orchards and using GenEluteTM Mammalian Genomic DNA Kit (Sigma-Aldrich, Germany) their genomic DNA was isolated. To assess genetic polymorphism 13 previously published microsatellite primer pairs developed for this pest were selected (Franck et al., 2005, Mol. Ecol. Notes 5: 99-102 and Zhou et al., 2005, Mol. Ecol. Notes 5: 226-227). Amplification of PCR products was performed as described by Franck et al. (2005) and Zhou et al. (2005) and visualization of fragments was done on 6% polyacrylamide gels followed by silver staining (Bassam and Caetano-Anolles, 1993, Appl. Biochem. Biotechnol. 42: 181-188). Four loci showed very good level of polymorphism (9 – 13 alleles per locus), eight loci showed satisfactory polymorphism (2 – 4 alleles per locus), while only one locus was monomorphic. Mean expected heterozygosity (HE) ranged from 0.01 to 0.84, while mean observed heterozygosity (HO) ranged from 0.01 to 1 for all loci across all individuals. This results give new insight on genetic structure of this economically important pest in Croatia.

Cydia pomonella L.; SSR markers; genetic polymorphism

nije evidentirano