engleski

GL Failure of bacteriophage typing to detect an iner-hospital outbreak of methicillin-resistant Staphylococcus aureus (MRSA) in Zagreb subsequently identified by random amplification of polymorphic DNA (RAPD) and pulsed-field gel electophoresis (PFGE).

Objective:To establish the extent of inter-hospital spread of methicillin-resistant Staphylococcus aureus (MRSA) in Zagreb and to determine the msot suitable method for typing local strains. Method: We analysed a collection of 33 MRSA isolaets from three zagreb hospitals tigether with unrelated British MRSA isolates by antibiogram typing, bacteriophage typing, randomly amplified polymorphic DNA (RAPD), analysis, and pulsed-field gel electrophoresis (PFGE) after digestion with SmaII restiction endonuclease. Bacteriopghage typing was done with international set of S.aureus typing phages. RAPD and PFGEprofiles were analysed visually and by using the "GelCompar" computer program. Results: Antibiogram typing provided eight profiles. Thirty (91%) of 33 Croatian strains of MRSA were nontypable by phage typing.Visula analysisi of RAPD products identified six, and visual analysisi of PFGE fragments nine distinct profiles. Computer analysis of RAPD data separated British isolates from Croatian ones, but did not cluster the visually determined RAPD types. PFGE computer analysis separated british analysis and clusterd isoaltes in concordance with visual interpretation Thirty-one of the 38 isolates (82%) were visually grouped in the same clusters by both molecular methods.The dominant strain was present in each of the three hospitals. Conslusions: Bacteriophage typing was unhelpful for the analysis of Croatian MRSA, since most strains were untypable with the international set of bacteriophages. RAPD and PFGE were more successful in typing the organisms and showed evidence of interhospital spread of one predominant MRSA strain in all three Zagreb hospitals. Thus RAPD and PFGE proved to be a useful aid in elucidating the epidmiology of MRSA infection in Zagreb hospitals and should be established in Croatia for typing MRSA.

Staphylococcus aureus (MRSA); polymorphic DNA (RAPD); pulsed-field gel electophoresis ( PFGE )

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