engleski

Mathematical modeling of poly[(R)-3-hydroxyalkanoate] synthesis by Cupriavidus necator DSM 545 on substrates stemming from biodiesel production

Two low structured mathematical models for fed-batch production of polyhydroxybutyrate and poly[hydroxybutyrate-co-hydroxyvalerate] by Cupriavidus necator DSM 545 on renewable substrates (glycerol and fatty acid methyl esters-FAME) combined with glucose and valeric acid, were established. The models were used for development/optimization of feeding strategies of carbon and nitrogen sources concerning PHA content and polymer/copolymer composition. Glycerol/glucose fermentation featured a max. specific growth rate of 0.171 h-1, a max. specific production rate of 0.038 h-1 and a PHB content of 64.5%, whereas the FAME/valeric acid fermentation resulted in a max. specific growth rate of 0.046 h-1, a max. specific production rate of 0.07 h-1 and 63.6 % PHBV content with 4.3% of 3-hydroxyvalerate (3HV) in PHBV. A strong inhibition of glycerol consumption by glucose in glycerol/glucose broth was confirmed (inhibition constant ki, G =4.28×10-4 g L-1). The direct proportionality between specific FAME content in biodiesel and the rate of consumption is indicated.

Cupriavidus necator; biodiesel; polyhydroxybutyrate (PHB); poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV); mathematical modeling

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