engleski

Molecular Characterization of Wild Grapevine (Vitis vinifera ssp. sylvestris) Populations of Northern Albania

Since 2003, research has been launched in order to identify, map, describe and characterize the populations of wild grapevine in northern Albania. In this study, three populations found in Mat, Drin and Shkreli valleys represented with total of 19 individual plants have been the subject of molecular characterization using 9 SSR markers. Relevant population statistics parameters such as average number of alleles, allele frequencies, He, Ho, PIC, as well as F-Statistics measures (FIS and FST) were calculated. Also, cluster analysis was carried out based on Dice similarity indices calculated for each possible pair-wise combination of analyzed genotypes. The number of observed alleles varied from five to eight (average 6.6) per locus. The most polymorphic loci were VVS2 and VVMD7 with majority of genotypes being heterozygous. Marker PIC values varied from 0.452-0.753 and the level of heterozygosity (Ho=0.696 ; He=0.656) was rather high compared to similar studies.

microsatellites; SSR markers; genotyping; genetic structure

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