engleski

Genetic polymorphism of paraoxonase and ApoE in coronary heart disease

Human serum paraoxonase, encoded by PON1 gene, is a high density lipoprotein (HDL) associated arylesterase. A common genetic polymorphism of the PON1 gene involves a glutamine (A allele) to arginine (B allele) interchange at position 192 (1). The molecular basis of apo E polymorphism are cysteine-arginine interchanges (E2: Cys 112/Cys 158, E3: Cys 112/Arg 158 and E4: Arg112/Arg 158). Polymorphic structural gene locus for apo E seems to be a major determinant of lipid concentrations and of the interindividual variations in susceptibility to vascular disease. The aim of this study was to determine genotype distribution for PON1 and Apo E in healthy Croatian population as well as the population of patients suffering from coronary heart disease (CHD). The genotyping was performed in 51 CHD patients and 70 healthy volunteers, on the whole blood DNA by PCR-RFLP (2). PON1 genotype frequencies differed significantly (P=0,017, c2-analysis), between patients and controls. Apo E genotype frequencies also differed between the patient and control group at the level of significance of P=0,064, as estimated by c2-analysis. Within the group of heterozygotes for Apo E (E2/3, E3/4) B allele frequency (46,7 %) was statistically different from the control group (P=0,009, c2-test). Our results indicate that both common PON1 and Apo E genetic polymorphisms might make some contribution to the CHD risk.

PON1; Apo E; coronary heart disease

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