Conformation of DNA in low added salt solutions (CROSBI ID 596543)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa
Podaci o odgovornosti
Grgičin, Danijel ; Dolanski Babić, Sanja ; Ivek, Tomislav ; Vuletić, Tomislav ; Tomić, Silvia
engleski
Conformation of DNA in low added salt solutions
Function of DNA in vivo is determined by its conformation, e.g. the transcription of DNA can not start if DNA is not in the single stranded (ss) form. We study influence of intrinsic DNA ions on conformation in low added salt limit (<0.02 mM). Although, this is an opposite limit, it is a step forward in understanding the DNA conformation in vivo. We use dielectric spectroscopy (DS) and UV spectrophotometry to study DNA semidilute solutions in the concentration range 0.01-5 g/L before and after the controlled denaturation. Denaturation was accomplished by heating the samples for 20 min at a temperature of 97°C, followed by quenching to 4°C. Measurements were subsequently made at 25°C. We focus on the question of minimal intrinsic concentration which keeps Mg-DNA in the native double stranded (ds) conformation. The results obtained are as follows. Mg-DNA is in ds conformation at least above the concentration c=0.01 g/L. Na-DNA is also in ds conformation, but below concentration c=0.5 g/L it shows locally exposed hydrophobic core in a dynamic sense [1]. [1] S.Tomić, S. Dolanski Babić, T. Vuletić, S. Krča, D.Ivanković, L. Griparić i R. Podgornik, Phys. Rev. E 75, 021905 (2007).
DNA; UV spectrophotometry; dielectric properties; dynamics and conformational changes; divalent counterions
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o prilogu
24-24.
2012.
objavljeno
Podaci o matičnoj publikaciji
7th Christmas Biophysics Workshop : Book of Abstracts
Pabst, Georg ; Heftberger, Peter ; Kollmitzer, Benjamin
Graz:
Podaci o skupu
7th Christmas Biophysics Workshop (XBW 2012)
predavanje
17.12.2012-18.12.2012
Riegersburg, austrija