engleski

Aqueous two-phase extraction of laccase from Trametes versicolor within microfabricated devices

Laccases are widely used enzymes especially in bioremediation, beverage (wine, fruit juice and beer) processing, ascorbic acid determination, sugar beet pectin gelation baking and as a biosensor [1]. Their activity has been extensively demonstrated in more than 60 fungal strains [2]. Several methods for purification of laccase have been reported (ion exchange chromatography, ultrafiltration, dialysis) where the multi-step process results in low yield with high cost of supplies and operation [3]. Compared to standard extraction processes with organic solvents aqueous two-phase system (ATPS) offers a number of advantages such as the non- denaturing conditions with high biocompatibility, low material cost, high yield, less energy consumption and shorter process time [4]. While it is difficult to uniformly reduce the size of the droplets in standard batch processes with ATPSs, for this reason microfluidic devices are well suited for speeding up the corresponding purification and separation processes. Usually, instead of having droplets of one phase in another, at least two streams of immiscible solutions are guided through a microchannel. This allows adjusting the length of the diffusion path by geometric constraints or hydrodynamic means. Process intensification due to acceleration of transport phenomena and the continuous flow within microfluidic devices is typically preferred over the batch preparations [5].

aqueous two-phase systems; laccase; extraction; microreactor

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