engleski

Characterization of endangered grapevine cultivars native to Croatia and Montenegro by SSR and S-SAP markers

In Croatia and Montenegro viticulture has a very long tradition and plays a significant role in the agricultural sector. Besides modern international varieties and well established native varieties such as Vranac and Plavac mali, there are numerous autochthonous underutilized cultivars that need to be properly conserved and evaluated. They might have potential in production of original local wines and having valuable genes to be used in breeding programs. The objectives of the present study were (1) to collect underutilized and endangered cultivars in Croatia and Montenegro, (2) to define their reliable identification key by molecular markers (SSRs), (3) to determine synonyms, homonyms or misslabeled accessions by comparing genotypes with available database, as well as (4) to assess genetic variability among vines originating from the same cultivar (clonal variation). Standard set of nine microsatellite (SSR) markers was used for genotype identification. A modified S-SAP (Sequence-Specific Amplified Polymorphism) method with universal primers for retrotransposons was used to study intravarietal variation of nine varieties. Total of 200 accessions from different locations in Croatia and Montenegro were collected and genotyped at nine SSR loci. Twenty-one different genotype was determined as well as number of duplicates for each genotype/variety. The overall similarity among accessions of the same variety was higher then 97, 5%, as expected with closely related accessions.

Grapevine; endagered cultivars; SSR; S-SAP

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