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An infrared insight into honeybee (Apis mellifera L.) haemolymph: analytical potentials

As functional equivalent to blood and lymph of vertebrates, insect haemolymph represents equally complex multifunctional system. Most of the records on honeybee haemolymph composition are focused on individual analysis of selected biochemical compounds, but it has not yet been explored on comprehensive molecular level. As opposed to standard biochemical methods, midium- infrared spectroscopy (MIR) provides a unique fingerprint of a sample based on overall biochemical composition (molecular structure) therefore enabling low-cost and multi-component measurement. Presence of unique features in IR spectrum that correspond to the specific functional groups in organic molecules is a major reason for broad acceptance of IR spectroscopy as analytical tool. Fourier transformed infrared (FT- IR) spectroscopy is a rapid and powerfool method utilized nowdays for analysis of various biological specimens (blood, tissues, microorganisms), which indicates on the possibility to detect alterations that occur in the haemolymph due to both physiological differences and pathological changes. However, this method has never been applied on any insect species in this regard. The feasibility study of FTIR-method for haemolymph analysis was based on 88 individual haemplymph samples of workers that were recorded using FT-IR attenuated total reflectance (ATR) technique. This study provides the first infrared insight into honeybee haemolymph by biochemical interpretation of the obtained FTIR spectra.

Apis mellifera L.; honeybee haemolymph; infrared spectroscopy; fingerprint

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