Distribution of metals in cytosol and physiological status of mussels as an estimation of trace metal exposure (CROSBI ID 597931)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa
Podaci o odgovornosti
Strižak, Željka ; Erk, Marijana
engleski
Distribution of metals in cytosol and physiological status of mussels as an estimation of trace metal exposure
The estimation of a trace metal exposure of the native mussel populations under influence of maritime activities using integration of biochemical and modern analytical techniques is the main subject of this study. Areas with a frequent nautic transport are affected by the trace metal pollution caused by leaching of antifouling paints which can have a permanent effect on biochemical and physiological processes in the organism. The earliest effects of the contaminants can be noticed at a cellular level and serve as biomarkers with a final goal of an effective environmental monitoring. Combining different approaches, e.g. a possibility of an organism to deal with the stress and specific trace metal responses on the cellular level can give a better final estimation of trace metal effects on the organism in the realistic environmental conditions. Therefore, two main approaches are applied on the Mediterranean mussel (Mytilus galloprovincialis) as a bioindicator organism using the digestive gland as a target tissue. General stress is estimated using a general physiological biomarker Cellular Energy Allocation (CEA) as an alternative approach to the Scope for growth (SfG). Since the metal binding to various cytosolic biomolecules as ligands represents a way of protection against the toxicity, an investigation of cytosolic metal distribution in protein fractions of different molecular masses is of a great importance for defining possible biomarkers of the trace metal exposure. This is achieved by size exclusion (SE) high performance liquid chromatography (HPLC) connected to inductively coupled plasma mass spectrometry (ICP-MS). The results will make a step towards the charachterisation of cytosolic metalloproteins responsible for metal handling in mussels under the realistic environmental exposure.
Mytilus galloprovincialis; cytosolic metals; cytosolic metalloproteins; CEA; SE-HPLC-ICP-MS
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Podaci o prilogu
93-93.
2013.
objavljeno
Podaci o matičnoj publikaciji
Interdisciplinary discourse on current environmental challenges
Melato, Aupaki Michael ; Brinkmann, Markus
Podaci o skupu
The 3rd Young Environmental Scientists Meeting
poster
11.02.2013-13.02.2013
Kraków, Poljska