engleski

Evaluation of an Osteoinductive Calcium-phosphate Ceramic with or without rhBMP-2 in Critical-sized Bone Defects of Rats

The aim of this study was to investigate the ability of an osteoinductive biphasic calcium phosphate ceramic (BCP) alone or in combination with rhBMP-2 to repair a 14 mm critical-sized calvarial bone defect in 120 male Wistar rats. Animals received a PTFE ring left empty (sham) or filled with BCP (BCP-0, Xpand Biotech, 150-500μm granules) consisting of β-tricalcium phosphate and hydroxyapatite in 80:20 ratio, BCP loaded with 2.5 (BCP -2.5) or 5.0 µg rhBMP-2 (BCP-5.0) per implant. Animals were sacrificed 3, 6, and 12 weeks post-implantation, respectively. Histomorphometric measurements were performed on Goldner trichrome-stained decalcified sections by software-assisted anaysis (Osteomeasure, Osteometrics, USA). New bone formation was expressed as area percentage of bone in the total available space (% BV/TV, mean±SD). Macroscopically, implants appeared firmly attached to the calvariae. In the sham group, new bone formation 3, 6, and 12 weeks post-implantation was 5.36±0.66, 4.66±1.10, and 6.80±1.90 respectively. In groups grafted with BCP newly formed bone extended from host bone bed to the center of the defect. At 3 weeks, % BV/TV was 14.73±0.65 in BCP-0, 17.41±1.34 in BCP-2.5, and 15.04±1.10 in BCP-5.0. At 6 weeks, a slight increase in new bone formation was observed – 23.92 ±1.60, 20.14±2.60 and 21.26±2.90 in BCP-0, BCP-2.5 and BCP-5.0, respectively. At 12 weeks % BV/TV was evaluated as 15.76±2.50, 20.41±0.90 and 20.35±1.70 for BCP-0, BCP-2.5, and BCP-5.0, respectively. No statistical differences among the groups were observed at any time point. These results demonstrate a comparable performance of an intrinsically osteoinductive ceramic alone or in presence of rhBMP-2 in regenerating critical-sized defects in rats.

BCP; bone defect; bone induction; rhBMP-2

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