engleski

In vitro detection of DNA damage in human leukocytes induced by combined effect of composite resins and adhesive systems

The aim of this study was to simultaneously evaluate the genotoxicity of dental composites and adhesive systems in vitro using a cytogenetic assay, with respect to the influence of composite shade. Genotoxicity assessment was carried out in human peripheral blood leukocytes using the comet assay. Three composite resin materials, two micro-hybrids and one nano-hybrid, in shade A1 and A3.5 were used with manufacturer-recommended four adhesive systems. Cultures were treated for 48 hours with samples after elusion for 1 hour, 1 day, 7 days or 30 days, in two different concentrations (4.16 mg/mL, 8.33 mg/mL). Kruskall-Wallis test was used for the statistical analysis (α=0.05). For combinations of micro-hybrid composite (A3.5) with two self-etch adhesives (16.1 ± 5.50 and 16.2 ± 9.52) after exposure to samples eluted for 1 day the incidence of primary DNA damage was significantly higher than for the corresponding negative control (14.7 ± 2.85). Genotoxicity was also higher after treatment with samples eluted for 1 hour (15.3 ± 4.70) and 1 day (15.3 ± 9.10), comprised of nano-hybrid composite (A1) with self-etch adhesive in relation to the control (13.1 ± 1.70). There was no clear trend of increased DNA damage in material combinations with darker shades of composites. Material composition and higher material concentrations showed greater influence on the genotoxicity. Considering effective biological repair mechanisms and the conditions of the present study, it can be concluded that darker shades of tested material combinations do not pose a significant risk for clinical application in terms of their biocompatibility.

adhesive systems; comet assay; composite resins; composite shade; genotoxicity; human leukocytes

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