engleski

Behavior of Campylobacterjejuni LBA65 and Campylobacter Bacteriophage PC5 in Mice Model

Campylobacters as leading cause of foodborne diseases are estimated to cause 9 million cases per year in EU. Because the number of campylobacteriosis cases is increasing since 2005, efficient methods for bio-sanitisation and therapeutic treatments are needed. Among novel antimicrobial agents, bacteriophages have shown to have a therapeutic and diagnostic potenitial. In the present study we investigated behaviour of C. jejuni and it's bacteriophage in the mice model. Campylobacter jejuni strain LBA65 has been isolated from poultry and it is a motile strain with two polar flagella and capsular polysaccharide. It has been shown that it is sensitive to many bacteriophages and also to flouroquinolone antibiotics, tetracycline and erythromycin. Virulence of C. jejuni LBA65 has been determined in Balb/c mice, infected intraperiotoneally with bacterial load ranging from 107 to 1010 CFU. Infected mice were sacrificed at different time points and the bacterial numbers in the spleen and liver homogenates were determined by plating out and Campylobacter specific quantitative PCR. In addition, local and systemic cytokine response during infection has been analysed. Using the same mice model we examined also stability and distribution of Campylobacter specific bacteriophage PC5. This bacteriophage belongs to Myoviridae family and requires bacterial capsule to initiate infection in LBA65. It's storage stability has been determined over one year long period. In vivo stability has been determined by intravenous infection of mice with 108 plaque forming units (PFU) of chromatographically purified bacteriophages. Changes in bacteriophage number has been followed for 12h by infectivity assay and PC5-specific quantitative PCR.

Campylobacter jejuni; Campylobacter Bacteriophage; virulence; mice

nije evidentirano