Modulation of Campylobacter jejuni Pathogenicity as a Consequence of Environmental Stress Response (CROSBI ID 599997)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Klančnik, Anja ; Vučković, Darinka ; Abram, Maja ; Smole Možina, Sonja
engleski
Modulation of Campylobacter jejuni Pathogenicity as a Consequence of Environmental Stress Response
Introduction: Campylobacters have developed a number of mechanisms for responding to environmental conditions. However, the different virulence properties of these cells following exposure to stress are still poorly understood. Methods: We analysed in vitro response to environmental stress (starvation, oxidative stress, heat shock) and the consequent modulation of Campylobacter jejuni pathogenicity in eukaryotic cell (Caco-2, J774 murine macrophages) and in vivo in a murine model. Results: In vitro, the influence of starvation and oxidative stress has milder effect than that of heat shock, although all of the stress conditions influenced the survival of C. jejuni. As published, we proved that environmental stresses have influenced the adhesion, invasion and intraepithelial survival of C. jejuni in eukaryotic cell models as well as the course of the infection of BALB/c mice. The systemic infection of mice occurred no matter what stress was investigated, but with different bacterial load in their livers and spleens as well as different production dynamics of the cytokines investigated (interleukins 6 and 10, tumor necrosis factor- α, interferon-γ) in plasma and liver homogenates. The most pronounced differences were in interferon-γ and interleukin 10 productions, indicating their roles in the immune response to C. jejuni infection. Impact of research: The study of environmental impact on bacterial virulence reveals that microbial adaptation during stress challenge is crucial not just for pathogen survival out of the host, but also during host-pathogen interactions, and thus for the bacterial pathogenicity.
Campylobacter jejuni; Pathogenicity; Mice; Stress response
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o prilogu
69-69.
2013.
nije evidentirano
objavljeno
Podaci o matičnoj publikaciji
Journal of Medical Microbiology
Forbes, K ; El Omar, E ; Strachan, N ; Hold, G
Aberdeen: Society for General Microbiology
0022-2615
Podaci o skupu
17th International Workshop on Campylobacter, Helicobacter and Related Organisms.
poster
15.09.2013-19.09.2013
Aberdeen, Ujedinjeno Kraljevstvo
Povezanost rada
Kliničke medicinske znanosti, Biotehnologija