engleski

EFFECT OF MIXTURE COMPOSED OF β GLUCANES AND MOS ON THE IMMUNOLOGICAL PARAMETERS IN THE REARED EUROPEAN SEA BASS

We used a mixture composed of yeast (Saccharomyces cerevisiae) that contains a blend of beta 1.3 and 1.6 glucans and MOS (mannan oligosaccharide), suggested to have beneficial effect when administered in fish feed at a rate of 0.1%. To our knowledge there are no studies that assessed the physiological response of the reared European sea bass (Dicentrarchus labrax) to the supplementation of feed with such immunostimulant mixture (IM). Moreover, practical trials on the farm have suggested that fish indeed exhibit better growth rate and resistance to the farm strains of Tenacibaculum maritimum. This for we have fed sea bass (N=60 per trial) for 4 weeks by 4 different feeding regimes: 1.) IM administered daily in feed by 0.1% ; 2.) IM administered in feed by 0.1% on every 4th day of feeding ; 3.) IM administered daily in feed by 0.1% for 2 weeks, followed by regular feed the next 2 weeks ; 4.) control fish fed by regular feed without IM. Prior to the feeding trial (day 0) and further on 1st, 4th, 7th, 14th, 21st and 28th day of trial, different physiological parameters were sampled: fish length and weight ; serum and whole blood for lysozyme activity and macrophage respiratory burst ; gills, gut and hematopoietic organs for histological analysis as well as for expression of interleukin-10. Our preliminary results show that although IM did not significantly affect fish growth, different feeding regimes induced different levels of innate immunity reaction.

European sea bass; Dicentrarchus labrax; immunostimulation

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