Use of γ-H2AX foci assay on human peripheral blood lymphocytes as sensitive biomarker of exposure (CROSBI ID 600702)
Prilog sa skupa u zborniku | izvorni znanstveni rad | domaća recenzija
Podaci o odgovornosti
Gerić, Marko ; Štraser, Alja ; Gajski, Goran ; Nunić, Jana ; Žegura, Bojana ; Filipič, Metka ; Garaj-Vrhovac, Vera
engleski
Use of γ-H2AX foci assay on human peripheral blood lymphocytes as sensitive biomarker of exposure
In modern medicine, it is impossible to imagine diagnostics and treatments without equipment that emit radiation (X-ray, CT, PET, etc.). At the same time there is a need to minimize the amount of radiation that the patient will gain during such medical examination. In that manner ALARA (As Low As Reasonably Achievable) principle and dosimetry are the bases of assuring patients safety. The induction of gamma phosphorylated H2AX histone is newly developed tool in biodosimetry, which is more sensitive for the detection of radiation caused DNA damage than currently used micronucleus and comet assay. Gamma phosphorylation of H2AX histone is a consequence of DNA double strand breaks and its role is to trigger the DNA repair mechanisms. In this study, we tested the effect of 2 and 4 Gy γ-radiations on human peripheral blood lymphocytes from two healthy volunteers using γH2AX foci assay. The FITC signal from labelled antibodies was monitored using flow cytometry and clearly demonstrated the difference in control samples and irradiated samples. There was also the difference between the exposed blood samples from the two volunteers. The results of present study reveal new sensitive method that is capable of detecting changes in DNA when exposed to different doses of radiation, and thus potentially optimizing the ALARA principle.
γ-H2AX foci assay; X-rays; lymphocytes
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Podaci o prilogu
166-171.
2013.
objavljeno
Podaci o matičnoj publikaciji
Zagreb:
978-953-96133-8-7
Podaci o skupu
9th Symposium of the Croatian Radiation Protection Association
poster
10.04.2013-12.04.2013
Krk, Hrvatska