engleski

Cytotoxic and genotoxic effects of water and sediment samples from gypsum mining area in channel catfish ovary(CCO)cells

Man-made activities such as mining generate certain amount sofmetal contaminated wastes which can reach aquatic environment and cause the serious effects on different organisms and ecosystem. Chemical analysis of the environmental samplesis the most direct approach to reveal their pollution status butt cannot always provide information on biological effects to different organisms, including fish. This study was aimed to investigate the in vitro cytotoxicity and genotoxicity of water and sediment sampl es from gypsum miningareau sing the channel cat fish ovary(CCO)cellline. Results obtained by the WST- assay and alkaline comet assay revealed that exposure of CCO cellsto the same concentrations of contaminated water and sediment samples caused significant decrease in cell viability and increased DNA damages. Chemical analysis of water and sediment samples showed that increas edconcentrations of strontium, aluminum and iron were mainly responsible for the observed cytotoxi candgenotoxiceffects in CCOcells. The study suggested that fish CCOcell scould be useful biological test-system for water an dsediment cytotoxicity and genotoxicity assessments.

Gypsum minewastewaters Comet assay CCO cells In vitro genotoxicity WST-1assay

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