engleski

Evaluation of high speed centrifugation and Lipoclear® reagent for lipemia removal

Background: Lipemic samples are a common problem in everyday laboratory practice ; however there are not many studies that have evaluated protocols for lipemia removal. The aim of this study was to evaluate the test recovery after lipid removal with high speed centrifugation and after processing the samples with commercially available Lipoclear® reagent. Materials and methods: Plasma pool was collected for the purpose this study. The samples were spiked with fat emulsion Intralipid® (Fresenius Kabi AB, Uppsala, Sweden) to create final concentrations of 300 and 500 mg/dL Intralipid®. Aliquotes of these lipemic samples were centrifuged at 12, 100 g for 5 minutes or processed with Lipoclear® reagent (Iris International, Inc., Westwood, MA, USA) according to the original manufacturer’s protocol. The following biochemical parameters were determined in native plasma, lipemic plasma and in samples after lipemia removal: bilirubin, direct bilirubin, urea, creatinine, glucose, sodium, potassium, chlorides, calcium, phosphates, magnesium, iron, AST, ALT, CK, CK-MB, LD, ALP, GGT, amylase, lipase, total protein, albumin, CRP, troponin T and proclacitonin. All measurements were performed in triplicates on Beckman Coulter AU680 analyzer (Tokio, Japan) using original reagents (Beckman Coulter, Inc., Brea, CA, USA), except for troponin T and procalcitonon which were measured on Cobas E411 analyzer (Roche Diagnostic, Mannheim, Germany) with the original Roche reagents. The recovery for each parameter after Lipoclear® and centrifugation was calculated and compared with desirable specification for imprecision (DSI) according to Ricos et al. (except for procalcitonin according to Barassi). Results: DSI criteria for recovery were not met for calcium, total protein, sodium and chlorides after high speed centrifugation and for glucose, calcium, phosphates, magnesium, sodium, potassium, chlorides, ALP, GGT, CK-MB, total protein, albumin and troponin T after using Lipoclear®. Overall, the lowest recovery was found for CRP (6.27%) after the use of Lipoclear®. Conclusions: As of our results, high speed centrifugation of lipemic samples is a more acceptable method for lipemia removal than the commercially available Lipoclear® reagent.

interference; lipemia; test recovery

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