engleski

Resveratrol reduces oxidative damage on rat liver caused by ochratoxin A and citrinin

Humans and animals are continuously exposed to mycotoxins ochratoxin A (OTA) and citrinin (CTN) which are often found together because they are produced by the same strains of molds . One of the possible mechanisms of their toxicity is the increase of reactive oxygen species and induction of oxidative stress. It is already known that one of the best known antioxidants is resveratrol (RSV) – a polyphenol found in grapes and red wine. The aim of our study was to check whether RSV treatment could decrease the oxidative stress in OTA and CTN treated animals. In this study adult male Wistar rats were given orally daily for 21 days with RSV and combination of RSV, OTA and CTN. The results were compared with those obtained in animals given the combination of OTA and CTN. . All animals were sacrificed 24 hours after the last treatment and liver tissue was collected and frozen until the analysis of malondialdehyde (MDA). The concentration of MDa in liver of rats treated with RSV was lower than in controls given 51mM NaHCO3 (72.40% of MDA concentration of controls). MDA concentration in liver of animals treated with mycotoxins and RSV was higher than in rats treated only with RSV (111.60%, compared to RSV treated), but lower than in control rats treated with 51mM NaHCO3 (80.80%, compared to controls). These results indicate that RSV may significantly reduce the oxidative lipid damage caused by OTA and CTN.

antioxidants; high performance liquid chromatography; mycotoxins; Wistar rats

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