engleski

Biphasic collagen – hydroxyapatite scaffold for treatment of osteochondral lesions of the knee – preliminary results of the sheep study

The aim of this study was to apply biphasic collagen – hydrohyapatite scaffold for the treatment of osteochondral defects in sheep, thus enabling an inductive environment for the cartilage restoration. 4 sheep were assigned to two treatment groups. All sheep underwent two surgical procedures. During the first procedure, chondral defects 4 mm in diameter were created on the weight bearing surfaces of both condyles of the right femur. After six weeks, all chondral defects were converted to osteochondral defects 6 mm in diameter and 5 mm deep. In one group (n=2) scaffolds tailored to fit the defect were implanted using a press-fit technique (scaffold group). Second group underwent defect conversion only (negative control). 6 weeks after treatment surgery, all sheep were sacrificed and tissue blocks were obtained for histology analysis. The samples were fixed in 4% buffered paraformaldehyde and decalcified in 15% EDTA. Paraffin blocks were sectioned at 5 µm and stained with hematoxylin- eosin, safranin O and picrosirius red. Immunofluorescent staining was performed against collagen type II. 6 weeks after implantation, fibrous tissue with abundant blood vessels was formed in the defects of both groups. However, biphasic scaffolds induced tissue reaction with numerous giant cells in the defects. The research leading to these results has received funding from the European Union's Seventh Framework Programme (FP7/2007- 2013) under grant agreement n°278807.

scaffold; collagen I; hydroxyapatite

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