Inhibition of genotoxic effects of UVC radiation on human keratinocyte HaCaT cells by Echinacea purpurea (L.) Moench herbal extract (CROSBI ID 606624)
Prilog sa skupa u zborniku | izvorni znanstveni rad | domaća recenzija
Podaci o odgovornosti
Šegvić Klarić, Maja ; Kosalec, Ivan ; Milić, Mirta ; Kopjar, Nevenka
engleski
Inhibition of genotoxic effects of UVC radiation on human keratinocyte HaCaT cells by Echinacea purpurea (L.) Moench herbal extract
Exposure of skin to ultraviolet (UV) radiation might provoke acute and chronic inflammation and oxidative stress which might cause DNA damage leading to skin photoaging and photocarcinogenesis. Previously we showed that Echinacea purpurea (L.) Moench (EH) extract, rich in phenolic acids, has protective effect on human blood lymphocytes exposed to UVC radiation. In this study we checked whether the pre-treatment of human keratinocyte HaCaT cells with lyophilisate of EH (1 and 10 mg/mL) could reduce or prevent primary DNA damage induced by UVC radiation (253.7 nm) in laboratory conditions. Prior to that experiment we examined cell viability using MTT test upon exposure to EH and UVC (30 and 60 min) alone and in combination. Primary DNA damage in HaCaT cells was studied using the alkaline comet assay. Exposure of cells to EH and UVC alone or EH in combination with UV radiation did not reduced cell viability. Opposite to that UV radiation (30 and 60 min) caused a significant increase in the level of primary DNA damage (P<0.001). Pre- treatment of cells with both concentrations of EH was not genotoxic to HaCaT cells. Only concentration of 1 mg/mL EH successfully protected the cells against the effects of 30 min exposure to UVC radiation. Positive results obtained in this study speak in favour of continuing the research on effectiveness of Echinacea purpurea preparations and their potential application in developing cosmetic products for skin protection.
Photoprotective effect; polyphenols; UVC radiation; keratinocytes
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Podaci o prilogu
153-158.
2013.
objavljeno
Podaci o matičnoj publikaciji
Zbornik radova 9. simpozija Hrvatskog društva za zaštitu od zračenja
Knežević, Željka ; Majer, Marija ; Bronić Krajcar, Ines
Zagreb: Hrvatsko društvo za zaštitu od zračenja
978-953-96133-8-7
Podaci o skupu
9. SIMPOZIJ HRVATSKOG DRUŠTVA ZA ZAŠTITU OD ZRAČENJA
poster
10.04.2013-12.04.2013
Krk, Hrvatska