A new look at SSB proteins (CROSBI ID 606693)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Paradžik, Tina ; Ivić, Nives ; Filić, Želimira ; Herron, Paul ; Manjasetty, Babu A. ; Luić, Marija ; Vujaklija, Dušica
engleski
A new look at SSB proteins
Streptomycetes are saprophytic soil bacteria that exhibit complex life cycle resembling those of eukaryotes, such as Fungi. The linear chromosome of Streptomyces coelicolor contains two paralogous genes encoding single- stranded DNA binding proteins, ssbA and ssbB. The functions of these SSBs (SSB-A and SSB-B) have not been studied so far. By disrupting these genes we have shown that ssbA gene is indispensable for cell viability, while ssbB participate in genome segregation during sporulation. Mutant phenotype has been restored by the introduction of single copy of ssbB gene. We also demonstrated differential expression of two ssb genes in S. coelicolor. During bacterial growth, gene ssbA is constantly expressed at higher level ; its expression is decreasing towards late stationary phase, while the expression of ssbB is at all-time low. In minimal medium the ssbB gene is significantly up regulated. Promoters for both genes are determined. Surprisingly, promoter of ssbB gene possesses unusual features and is active in taxonomically distant bacteria Escherichia coli. The solved crystal structure of SSB-S protein reveals a common conserved OB-fold, lack of clamp like structure and previously unpublished S-S bridges. Further, comparative sequence analysis of SSB proteins from Actinobacteria was also performed revealing frequent duplication of ssb gene. Strong retention of ssb genes could be result of adoption of important cellular function.
Streptomyces ; paralogous ssb genes ; chromosomal segregation ; crystal structures
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o prilogu
49-49.
2012.
objavljeno
Podaci o matičnoj publikaciji
Actinobacteria within soils, Capacities for mutualism symbiosis pathogenesis
Schrempf, Hilde ; Ortiz de Orue Lucana, Dario
Osnabrück: Sveučilište Osnabrueck
Podaci o skupu
Actinobacteria within soils
pozvano predavanje
25.10.2012-28.10.2012
Münster, Njemačka