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Apoptosis and sperm DNA fragmentation index in native and processed sperm samples

Sperm preparation techniques are an essential component of assisted reproductive technologies, and they are related to the success of infertility treatment. The aim of this study was to compare apoptosis and sperm DNA fragmentation between native and processed (after density gradient) sperm samples, and to explore the relation between apoptosis and DNA fragmentation in both sperm samples. The participants were 30 men undergoing fertility treatments in a public hospital. The results showed significantly higher percentage of apoptosis in native sperm sample than in processed sperm sample. The percentage of DNA fragmentation did not differ between native and processed sperm samples. The relation between apoptosis and DNA fragmentation in processed sperm sample and native sperm sample was very low and did not reach statistical significance. Apoptosis was not significantly associated with the number of normal and fragmented forms of sperm cells and this pattern of relations was the same in both samples. The results showed that the age of patients was positively and significantly correlate to the value of apoptosis in processed sperm sample but not in native sample. In conclusion, the findings suggest that even in processed sperm samples the incidence of DNA fragmentation remains unchanged, although sperm processing results in a lower incidence of apoptosis especially in younger men. These findings point to importance of evaluation of apoptosis in processing sperm in IVF.

apoptosis; DNA fragmentation; sperm preparation technique

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