Reflectance colorimetry as a simple method for estimating carotenoid content in corn grain (CROSBI ID 202968)
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Podaci o odgovornosti
Kljak, Kristina ; Grbeša, Darko ; Karolyi, Danijel
engleski
Reflectance colorimetry as a simple method for estimating carotenoid content in corn grain
Yellow corn grain is the only cereal containing appreciable amounts of carotenoids, compounds that can act as antioxidants, pigments or vitamin A precursors. Current methods for determining carotenoids in corn grain are costly and time-consuming, so the aim of the present research was to evaluate whether simple grain color measurement might serve as a fast, low-cost and reliable method for predicting carotenoid content. The six commercial corn hybrids were analyzed for carotenoid profile using HPLC and color parameters using reflectance colorimetry (CIE L*a*b*). The hybrids varied substantially in carotenoid content and color parameters. Levels of zeaxanthin correlated positively with redness (a*) and hue angle (hab), while levels of lutein, β-cryptoxanthin and β-carotene correlated positively with yellowness (b*) and chroma value (Cab*). The level of total carotenoids correlated positively with all color parameters except hab. Multiple linear regression was carried out in which scalar color coordinates (L*, a* and b*) and angular coordinates (L*, hab and Cab*) were considered independent variables, and equations with R2 > 0.9 were obtained. The results show that individual and total carotenoid content of corn grain can be accurately predicted using colorimetry, making it a fast and inexpensive method for carotenoid estimation in this crop.
corn grain; carotenoids; reflectance colorimetry; CIE L*a*b*
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