engleski

Determining the identity of a promising new sour cherry cultivar using SSR markers

The genetic identity of a promising new sour cherry (Prunus cerasus) cultivar ‘elika’ was examined using seven microsatellite markers developed from peach (Prunus persica). Along with the previously mentioned cultivar, four international, commercial cultivars (‘Schatten morelle’, ‘Rexelle’, ‘Doubbele Gorsem Kreiek', 'Heimanns Konservenweichsel') and two most commonly grown regional cultivars (‘Maraska’ and ‘Oblainska’) were also analyzed. Seven primer pairs managed to amplify 46 different alleles, as well as distinguish all the analyzed genotypes. UPGMA dendrogram based on genetic similarity (Dice) was constructed in order to examine the relationships of the analyzed cultivars. ‘Rexelle’, ‘Doubbele Gorsem Kreiek', 'Heimanns Konservenweichsel' clustered tightly with ‘Schatten morelle’, which is not surprising considering the fact that these three cultivars are supposed spontaneous seedlings of ‘Schatten morelle’. The new cultivar ‘elika’ also displayed very high level of genetic similarity with ‘Schatten morelle’ indicating that even this new genotype might be a spontaneous seedling of the famous cultivar. The cluster analysis showed that the examined ‘Maraska’ and ‘Oblainska’ genotypes grouped very closely together. This may indicate a recent common ancestor or hybridization events, especially since ‘Maraska’ and ‘Oblainska’ fit more the description of populations than that of classic cultivars.

Sour cherry; microsatellite; genetic similarity

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