engleski

EXPRESSION OF CADHERIN-CATENIN COMPLEX IN CLEAR CELL RENAL CELL CARCINOMA

Renal cell carcinoma (RCC) accounts for approximately 3% of all adult malignancies with the clear-cell type (CC-RCC) comprising 80% of RCC. Currently, prediction of patient survival is based on traditional pathomorphological parameters, including tumor size, Fuhrman nuclear grade and UICC pTN classification. The cadherin-catenin complex influences migration and modifies cell growth and survival of neoplastic cells. The aim of this study was to determine immunohistochemical expression of E-cadherin, N-cadherin and beta-catenin in CC-RCC and compare it with currently used prognostic parameters. Forty-two paraffin embedded tumor samples of CC-RCC from the archive of Ljudevit Jurak University Department of Pathology were examined. For each case, the percentage of stained tumor cells was estimated for all three markers and the staining pattern was also noted. MedCalc software (Mariakerke, Belgium) was used for statistical analyses. Results were analyzed using Mann-Whitney test and Spearman's rank correlation coefficient ; p<0.05 was considered to be statistically significant. At the time of diagnosis, the median patient age was 65.4 (range 46-80) years, with tumor size median of 3.3 (range 0.9-8.0) cm. All samples of CC-RCC showed positive membrane immunohistochemical reaction for E-cadherin (median 4.98%) and N-cadherin (median 25.48). Staining for beta-catenin was membranous (median 20.36%) and cytoplasmic (median 20.60%). The relationship between membranous beta-catenin and membranous N-cadherin and cytoplasmic beta-catenin and membaranous N-cadherin was significant (p=0.026 and p=0.001, respectively). Comparison of the prognostic factors with the immunohistochemical expression of study markers showed that there was significant positive correlation between pT stage and expression of E cadherin, as well as between tumor size and N-cadherin expression. In conclusion, our study showed a correlation of N-cadherin and beta-catenin expression, indicating the potential role of N-cadherin in tumor progression

renal cell carcinoma

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