hrvatski

Biomonitoring of genotoxic and cytotoxic effects of buccal epithelial cells exposed to resin composite dental materials

Objectives: Dental composite materials come into direct contact with oral tissue. Due to this close and long-term contact, the materials should exhibit a high degree of biocompatibility. The micronucleus assay in human buccal epithelial cells is a useful and minimally invasive method for monitoring genetic damage in humans. This study was performed to evaluate possible DNA- damage to human buccal epithelial cells exposed to resin composite dental materials. Materials and Methods: The study comprises 80 young individuals (age range 22-25) ; 57 with composite fillings and 23 without composite restorations (control). Except oral status, demographic data and personal history of all subjects have been recorded. Buccal cells of oral mucosa have been obtained by gentle scraping with a cytobrush and processed for further analysis. 2000 epithelium cells per preparation were observed under a light microscope. Micronuclei and degenerative nuclear alterations (binuclear cells, pyknosis, karyolysis, karyorrhexis, nuclear bud, and nucleoplasmatic bridges, ) have been scored. Results: The results obtained show that the number of cavity fillings was not relevant in explaining the occurrence of micronuclei. The number of cavity fillings was a significant factor only in explaining the number of binuclear cells (p=0.065). The percent increase in the expected number of binuclear cells was 1% for every unit increase in the number of cavity fillings (p=0.061). Conclusions: Composite resin materials exhibited limited genotoxic and cytotoxic activity in buccal epithelial cells. Observed effects could not be indicated as biologically relevant and it should not pose a significant risk to the human genome. As dental composite resins remain in immediate contact with oral tissue over a long period of time, further research on their possible genotoxicity is advisable.

biocompatibility ; dental composite materials ; genotoxicity ; micronucleus assay

nije evidentirano

engleski

Biomonitoring of genotoxic and cytotoxic effects of buccal epithelial cells exposed to resin composite dental materials

Objectives: Dental composite materials come into direct contact with oral tissue. Due to this close and long-term contact, the materials should exhibit a high degree of biocompatibility. The micronucleus assay in human buccal epithelial cells is a useful and minimally invasive method for monitoring genetic damage in humans. This study was performed to evaluate possible DNA- damage to human buccal epithelial cells exposed to resin composite dental materials. Materials and Methods: The study comprises 80 young individuals (age range 22-25) ; 57 with composite fillings and 23 without composite restorations (control). Except oral status, demographic data and personal history of all subjects have been recorded. Buccal cells of oral mucosa have been obtained by gentle scraping with a cytobrush and processed for further analysis. 2000 epithelium cells per preparation were observed under a light microscope. Micronuclei and degenerative nuclear alterations (binuclear cells, pyknosis, karyolysis, karyorrhexis, nuclear bud, and nucleoplasmatic bridges, ) have been scored. Results: The results obtained show that the number of cavity fillings was not relevant in explaining the occurrence of micronuclei. The number of cavity fillings was a significant factor only in explaining the number of binuclear cells (p=0.065). The percent increase in the expected number of binuclear cells was 1% for every unit increase in the number of cavity fillings (p=0.061). Conclusions: Composite resin materials exhibited limited genotoxic and cytotoxic activity in buccal epithelial cells. Observed effects could not be indicated as biologically relevant and it should not pose a significant risk to the human genome. As dental composite resins remain in immediate contact with oral tissue over a long period of time, further research on their possible genotoxicity is advisable.

biocompatibility ; dental composite materials ; genotoxicity ; micronucleus assay

nije evidentirano